THE ROLE OF FTO GENE POLYMORPHISM IN CARDIAC AUTONOMIC NEUROPATHY AMONG INDIVIDUALS OF KAZAKH POPULATION

Abstract

In the pathogenesis of cardiac autonomic neuropathy, disruptions in lipid metabolism occur, which subsequently influence the reduction of neural blood flow, leading to decreased conductivity in the heart. It is commonly believed that the fat mass and obesity-associatedFTO gene is a predisposing gene for obesity, and its polymorphism rs12149832 plays a crucial role in obesity processes, particularly among the Asian population. However, as of today, there is a lack of studies examining the association of this polymorphism with cardiac autonomic neuropathy in the Kazakh population. The aim: To investigate the association between the rs12149832 polymorphism of the FTO gene and cardiac autonomic neuropathy among individuals of Kazakh population. Materials and Methods: A case-control study included 147 patients with cardiac autonomic neuropathy (cases) and 153 patients without cardiac autonomic neuropathy (controls). 300 individuals of Kazakh population were recruited from a hospital affiliated with the RSE "Medical Center Hospital of the President's Affairs Administration of the Republic of Kazakhstan." Genotyping for five polymorphisms of FTO gene was performed for all patients using real-time PCR. Statistical analysis involved Chi-square methods, calculation of odds ratios (OR) with 95% confidence intervals (CI). Statistical analysis was performed in a gene calculator Gen Expert. Results: As a result of preliminary genetic analysis, we found that of the 5 FTO gene polymorphisms, only the rs12149832 polymorphism was associated with cardiac autonomic neuropathy. In logistic regression analysis, rs12149832 polymorphism was associated with cardiac neuropathy in the group of patients without diabetes mellitus (2.98 (1.11-8.03) and 3.66 (1.24-10.77), respectively), while it was not associated in the overall group and the group with diabetes mellitus, both before and after adjustment for age, gender, total cholesterol, BMI, TG, HDL, LDL, glucose. Conclusion: Therefore, thers 12149832 polymorphism of the FTO gene is associated with a predisposition to cardiac autonomic neuropathy. Further research aimed at exploring the relationship of this polymorphism with cardiac neuropathy in conjunction with lipid metabolism indicators would allow for an assessment of its impact on the development of cardiac autonomic neuropathy.